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normal human colon mucosal epithelial cell line ncm460  (Keygen Biotech)

 
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    Keygen Biotech normal human colon mucosal epithelial cell line ncm460
    (A) IC 50 values of HCT-8/V and parental HCT-8 cells for vincristine, taxol, and cisplatin; RI represents the resistance index. (B) Rh123 efflux was measured using flow cytometry. (C) Western blot analysis of ABCB1 and ABCC1; GAPDH was used as a loading control. (D) Increased actin microfilament was observed in the HCT-8/V cells by cytoskeleton staining. (E) Immunofluorescence staining of <t>epithelial</t> and mesenchymal markers in HCT-8 and HCT-8/V cells. Cells were double-stained for vimentin (red) and E-cadherin (green). Nuclei were counterstained blue with DAPI. (F) Expression of EMT markers and transcription factors was determined by Western blot. GAPDH was used as a loading control. Scale bars represent 10 μm in D and E . Data from three independent experiments were graphed as the mean ± SD. * P < 0.05.
    Normal Human Colon Mucosal Epithelial Cell Line Ncm460, supplied by Keygen Biotech, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/normal+human+colon+mucosal+epithelial+cell+line+ncm460/ncm460+cells/pmc05581080-124-2-35
    Average 90 stars, based on 1 article reviews
    normal human colon mucosal epithelial cell line ncm460 - by Bioz Stars, 2026-09
    90/100 stars

    Images

    1) Product Images from "Twist1 confers multidrug resistance in colon cancer through upregulation of ATP-binding cassette transporters"

    Article Title: Twist1 confers multidrug resistance in colon cancer through upregulation of ATP-binding cassette transporters

    Journal: Oncotarget

    doi: 10.18632/oncotarget.17548

    (A) IC 50 values of HCT-8/V and parental HCT-8 cells for vincristine, taxol, and cisplatin; RI represents the resistance index. (B) Rh123 efflux was measured using flow cytometry. (C) Western blot analysis of ABCB1 and ABCC1; GAPDH was used as a loading control. (D) Increased actin microfilament was observed in the HCT-8/V cells by cytoskeleton staining. (E) Immunofluorescence staining of epithelial and mesenchymal markers in HCT-8 and HCT-8/V cells. Cells were double-stained for vimentin (red) and E-cadherin (green). Nuclei were counterstained blue with DAPI. (F) Expression of EMT markers and transcription factors was determined by Western blot. GAPDH was used as a loading control. Scale bars represent 10 μm in D and E . Data from three independent experiments were graphed as the mean ± SD. * P < 0.05.
    Figure Legend Snippet: (A) IC 50 values of HCT-8/V and parental HCT-8 cells for vincristine, taxol, and cisplatin; RI represents the resistance index. (B) Rh123 efflux was measured using flow cytometry. (C) Western blot analysis of ABCB1 and ABCC1; GAPDH was used as a loading control. (D) Increased actin microfilament was observed in the HCT-8/V cells by cytoskeleton staining. (E) Immunofluorescence staining of epithelial and mesenchymal markers in HCT-8 and HCT-8/V cells. Cells were double-stained for vimentin (red) and E-cadherin (green). Nuclei were counterstained blue with DAPI. (F) Expression of EMT markers and transcription factors was determined by Western blot. GAPDH was used as a loading control. Scale bars represent 10 μm in D and E . Data from three independent experiments were graphed as the mean ± SD. * P < 0.05.

    Techniques Used: Flow Cytometry, Western Blot, Control, Staining, Immunofluorescence, Expressing

    Related Articles

    Flow Cytometry:

    Article Title: Twist1 confers multidrug resistance in colon cancer through upregulation of ATP-binding cassette transporters
    Article Snippet: The normal human colon mucosal epithelial cell line (NCM460), the low invasive human colon adenocarcinoma cell line (HT-29), the highly invasive human colon cancer cells (HCT-8), HCT-8/V and Bel7402 hepatocarcinoma cell line were purchased from KeyGEN BioTECH (Nanjing, China). .. A

    Western Blot:

    Article Title: Twist1 confers multidrug resistance in colon cancer through upregulation of ATP-binding cassette transporters
    Article Snippet: The normal human colon mucosal epithelial cell line (NCM460), the low invasive human colon adenocarcinoma cell line (HT-29), the highly invasive human colon cancer cells (HCT-8), HCT-8/V and Bel7402 hepatocarcinoma cell line were purchased from KeyGEN BioTECH (Nanjing, China). .. A

    Control:

    Article Title: Twist1 confers multidrug resistance in colon cancer through upregulation of ATP-binding cassette transporters
    Article Snippet: The normal human colon mucosal epithelial cell line (NCM460), the low invasive human colon adenocarcinoma cell line (HT-29), the highly invasive human colon cancer cells (HCT-8), HCT-8/V and Bel7402 hepatocarcinoma cell line were purchased from KeyGEN BioTECH (Nanjing, China). .. A

    Staining:

    Article Title: Twist1 confers multidrug resistance in colon cancer through upregulation of ATP-binding cassette transporters
    Article Snippet: The normal human colon mucosal epithelial cell line (NCM460), the low invasive human colon adenocarcinoma cell line (HT-29), the highly invasive human colon cancer cells (HCT-8), HCT-8/V and Bel7402 hepatocarcinoma cell line were purchased from KeyGEN BioTECH (Nanjing, China). .. A

    Immunofluorescence:

    Article Title: Twist1 confers multidrug resistance in colon cancer through upregulation of ATP-binding cassette transporters
    Article Snippet: The normal human colon mucosal epithelial cell line (NCM460), the low invasive human colon adenocarcinoma cell line (HT-29), the highly invasive human colon cancer cells (HCT-8), HCT-8/V and Bel7402 hepatocarcinoma cell line were purchased from KeyGEN BioTECH (Nanjing, China). .. A

    Expressing:

    Article Title: Twist1 confers multidrug resistance in colon cancer through upregulation of ATP-binding cassette transporters
    Article Snippet: The normal human colon mucosal epithelial cell line (NCM460), the low invasive human colon adenocarcinoma cell line (HT-29), the highly invasive human colon cancer cells (HCT-8), HCT-8/V and Bel7402 hepatocarcinoma cell line were purchased from KeyGEN BioTECH (Nanjing, China). .. A



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    Image Search Results


    (A) IC 50 values of HCT-8/V and parental HCT-8 cells for vincristine, taxol, and cisplatin; RI represents the resistance index. (B) Rh123 efflux was measured using flow cytometry. (C) Western blot analysis of ABCB1 and ABCC1; GAPDH was used as a loading control. (D) Increased actin microfilament was observed in the HCT-8/V cells by cytoskeleton staining. (E) Immunofluorescence staining of epithelial and mesenchymal markers in HCT-8 and HCT-8/V cells. Cells were double-stained for vimentin (red) and E-cadherin (green). Nuclei were counterstained blue with DAPI. (F) Expression of EMT markers and transcription factors was determined by Western blot. GAPDH was used as a loading control. Scale bars represent 10 μm in D and E . Data from three independent experiments were graphed as the mean ± SD. * P < 0.05.

    Journal: Oncotarget

    Article Title: Twist1 confers multidrug resistance in colon cancer through upregulation of ATP-binding cassette transporters

    doi: 10.18632/oncotarget.17548

    Figure Lengend Snippet: (A) IC 50 values of HCT-8/V and parental HCT-8 cells for vincristine, taxol, and cisplatin; RI represents the resistance index. (B) Rh123 efflux was measured using flow cytometry. (C) Western blot analysis of ABCB1 and ABCC1; GAPDH was used as a loading control. (D) Increased actin microfilament was observed in the HCT-8/V cells by cytoskeleton staining. (E) Immunofluorescence staining of epithelial and mesenchymal markers in HCT-8 and HCT-8/V cells. Cells were double-stained for vimentin (red) and E-cadherin (green). Nuclei were counterstained blue with DAPI. (F) Expression of EMT markers and transcription factors was determined by Western blot. GAPDH was used as a loading control. Scale bars represent 10 μm in D and E . Data from three independent experiments were graphed as the mean ± SD. * P < 0.05.

    Article Snippet: The normal human colon mucosal epithelial cell line (NCM460), the low invasive human colon adenocarcinoma cell line (HT-29), the highly invasive human colon cancer cells (HCT-8), HCT-8/V and Bel7402 hepatocarcinoma cell line were purchased from KeyGEN BioTECH (Nanjing, China).

    Techniques: Flow Cytometry, Western Blot, Control, Staining, Immunofluorescence, Expressing